During the World Cup final people from 172 countries tweeted in 27 different languages

"The World Cup final represented the largest period of sustained activity for an event in Twitter’s history.
Throughout the match, Tweets-per-second (TPS) were much higher than average; during the game’s final 15 minutes, this jumped to more than 2,000 TPS. (Spain’s winning goal in the final scored a 3,051 TPS.)
During the final, people from 172 countries tweeted in 27 different languages.
At the moment of the winning goal, people from 81 countries tweeted in 23 different languages. This moment is represented on this Wordle infographic."
Source:  Twitter blog, 15th July 2010